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massarray epityper system (agena’s massarray epityper system)  (agena bioscience)


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    agena bioscience massarray epityper system (agena’s massarray epityper system)
    Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on <t>MassARRAY</t> (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
    Massarray Epityper System (Agena’s Massarray Epityper System), supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agena%E2%80%99s+massarray+epityper+system/massarray+epityper/pmc09223562-170-11-14
    Average 90 stars, based on 1 article reviews
    massarray epityper system (agena’s massarray epityper system) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions"

    Article Title: DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23126632

    Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
    Figure Legend Snippet: Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).

    Techniques Used: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Concentration Assay

    Related Articles

    Methylation:

    Article Title: Effects of maternal feed intake restriction on the blood parameters, fatty acid profile and lipogenetic genes expression of perirenal fat in offspring kids.
    Article Snippet: Maternal nutritional restriction impacted lipogenic gene expression in adipose tissue of offspring, but the association of this programming with DNA methylation is not yet clear.. Therefore, this experiment aimed to investigate the effects of maternal feed intake restriction on offsprings’ blood indexes, lipid metabolism of perirenal adipose tissue (PAT) and DNA methylation of lipogenic genes.. Blood and PAT were collected from the fetuses (at d 100 of gestation) and kids (at d 90 after birth).

    Mass Spectrometry:

    Article Title: Effects of maternal feed intake restriction on the blood parameters, fatty acid profile and lipogenetic genes expression of perirenal fat in offspring kids.
    Article Snippet: Maternal nutritional restriction impacted lipogenic gene expression in adipose tissue of offspring, but the association of this programming with DNA methylation is not yet clear.. Therefore, this experiment aimed to investigate the effects of maternal feed intake restriction on offsprings’ blood indexes, lipid metabolism of perirenal adipose tissue (PAT) and DNA methylation of lipogenic genes.. Blood and PAT were collected from the fetuses (at d 100 of gestation) and kids (at d 90 after birth).



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    Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on <t>MassARRAY</t> (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
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    Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on <t>MassARRAY</t> (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
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    Image Search Results


    Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).

    Journal: International Journal of Molecular Sciences

    Article Title: DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions

    doi: 10.3390/ijms23126632

    Figure Lengend Snippet: Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).

    Article Snippet: Quantitative methylation analysis of bisulfite-treated genomic DNA was conducted through Agena’s MassARRAY EpiTYPER system (Agena Bioscience, San Diego, CA, USA).

    Techniques: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Concentration Assay